A new research article has been published

We have published a new research article in Communications Biology, a newly launched journal from Springer Nature.

We have developed a system of automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer, called LiLACK (Laser-induced, Light-responsive- polymer-Activated, Cell Killing).

Fig. 1

We have demonstrated that human induced pluripotent stem cells (hiPSCs) maintained their pluripotency and self-renewal during long-term culture using this system in each passage for sectioning. Furthermore, combined with deep machine-learning analysis on fluorescent and phase contrast images, we have developed a label-free and automatic cell processing system by eliminating unwanted spontaneously differentiated cells in undifferentiated hiPSC culture conditions.

 

 

Full article can be read below the link (open access)

Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Yohei Hayashi, Junichi Matsumoto, Shohei Kumagai, Kana Morishita, Long Xiang, Yohei Kobori, Seiji Hori, Masami Suzuki, Toshiyuki Kanamori, Kazuhiro Hotta & Kimio Sumaru
Communications Biologyvolume 1, Article number: 218 (2018)