Published a co-authored article

From my previous laboratory (Gene regulation laboratory in University of Tsukuba),
an article titled “Live cell imaging of X chromosome reactivation during somatic cell reprogramming” has been published.

We generated female mouse embryonic stem cells (ESCs) that carry two-colored fluorescent protein expression on both alleles in X chromosomes. The ESC clones, which initially expressed both EGFP and hKO, inactivated one of the fluorescent protein genes upon differentiation, indicating that the EGFP and hKO genes are subject to X chromosome inactivation (XCI). When the derived somatic cells carrying the EGFP gene on the inactive X chromosome (Xi) were reprogrammed into iPSCs, the EGFP gene on the Xi was reactivated when pluripotency marker genes were induced. Thus, the fluorescent protein genes inserted into an intergenic locus on both X chromosomes enable live cell monitoring of XCI during ESC differentiation and XCR during reprogramming. This is the first study that succeeded live cell imaging of XCR during reprogramming.

Congratulations, Yen!