2. Generation of modified iPSC lines

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We modify iPSC lines deposited in and provided by RIKEN cell bank in order to enhance their usefulness. The type and methods of the modification are as follows:

  • We generate isogenic control cells by correcting specific mutations responsible for a disease using genome editing technology.
  • We generate mutation-introduced iPSC lines if the responsible genes are identified, but the number of disease-specific iPSC lines is not enough to be examined. We use healthy-donor iPSC lines provided by the RIKEN cell bank as the original iPSC lines for this purpose.
  • We generate reporter-introduced iPSC lines from disease-specific or healthy-donor iPSC lined with in order to monitor the differentiation status visually by using transgenic or knock-in to tissue or cell type specific promoters with fluorescent proteins.