2. Generation of modified iPSC lines

We modify iPSC lines deposited in and provided by RIKEN cell bank in order to enhance their usefulness. The type and methods of the modification are as follows:

  • We generate isogenic control cells by correcting specific mutations responsible for a disease using genome editing technology.
  • We generate mutation-introduced iPSC lines if the responsible genes are identified, but the number of disease-specific iPSC lines is not enough to be examined. We use healthy-donor iPSC lines provided by the RIKEN cell bank as the original iPSC lines for this purpose.
  • We generate reporter-introduced iPSC lines from disease-specific or healthy-donor iPSC lined with in order to monitor the differentiation status visually by using transgenic or knock-in to tissue or cell type specific promoters with fluorescent proteins.